pathscan® intracellular signaling array kit #7323  (Cell Signaling Technology Inc)

Journal: Parasitology Research

Article Title: Tick salivary cystatin Iristatin limits the virus replication in skin of tick-borne encephalitis virus–infected mice

doi: 10.1007/s00436-024-08441-5

Figure Lengend Snippet: Iristatin interferes with signaling pathways activation and exerts anti-apoptotic effect in TBEV-infected dendritic cells. DC were activated by imiquimod (IQ; 2 µg/ml) for 3 h in the presence or absence of Iristatin (3 µM) and protein cell lysates were analyzed for the activation of signaling pathways using PathScan intracellular signaling array ( a ). DC were infected by Hypr at MOI 5 for indicated times in the presence or absence of Iristatin (3 µM) and then Erk1/2 phosphorylation was analyzed by immunoblotting. Membranes were re-probed to determine the level of total Erk1/2 proteins. Proteins were visualized by chemiluminescence and representative blot with relative phosphorylation is shown ( b ). DC were non-infected or infected by Hypr at MOI 5 in the presence or absence of Iristatin (6 µM) and the percentage of active caspase-3 positive cells was measured by flow cytometry ( c ). * p ≤ 0.05; **** p ≤ 0.0001 ns = not significant

Article Snippet: After 3 h, the cell protein lysates were prepared and analyzed using the PathScan® intracellular signaling array kit (#7323, Cell Signaling Technology) according to the manufacturer’s instruction.

Techniques: Protein-Protein interactions, Activation Assay, Infection, Phospho-proteomics, Western Blot, Flow Cytometry